Abstract: Fascioliasis is one of the water and food born zoonotic disease caused by Fasciola hepatica, spread by various sources particularly containing water through various organisms ultimately to humans. The present study determines the prevalence of Fasciola hepatica in domestic animals (cow, sheep and goat) in district Karak, Khyber Pakhtunkhwa, Pakistan, from January through July, 2013. The study area is located at located 70-40° to71-30° North latitude and 32-48° to 33-23° East longitudes, with the mean annual precipitation over 300 mm. A total of 150 animals (cow, sheep, goat) were selected from the specified areas of district Karak and liver samples of 70 cows, 50 sheep and 30 goats were collected. The collected samples were preserved in 10% formalin, stained with borax carmin-ethanol for microscopic observations. The overall prevalence of the pathogen was 24.2, 16 and 6.6% in cow, sheep and goat, respectively. Monthly dataset showed the highest prevalence recorded in June (40.7%), while the minimum was in January. Cluster analysis showed the frequency was high in B.D. Shah region than Karak and Takht-E-Nasrati. This study will provide a base for further related research and aware the people about fascioliasis.