Abstract: Genotype has a significant effect on biological efficiencies of dairy cows. Environmental changes affect differently between different genotypes of animals. A biometrical study was conducted on reproductive organs of dairy cows (n = 100) of different genotypes (Local, Local×Holstein Friesian, Local×Jersey and Local×Sahiwal) available in Bangladesh. Reproductive tracts of cows were collected immediately after slaughter from different slaughter houses in Rajshahi, Bangladesh. The comparison of morphometric values of most of the parameters of reproductive tract observed in different genotypic cows showed significant differences (p<0.05). Local×Holstein Friesian had significantly higher values (p<0.05) on the most of parameters of genitalia measured, followed by the Local×Jersey, Local×Sahiwal and Local. Local×Holstein Friesian had the longest uterine horn (29.20±1.65 cm for right and 29.87±1.75 cm for left), uterine body (3.42±0.20 cm length and 2.83±1.40 cm width), cervix (5.64±0.24 cm length and 4.89±0.23 cm width) and vagina (24.66±0.64 cm length and 6.08±0.36 cm width). The right ovary was wider in diameter, larger in length and heavier in weight as compared to left one in all genotypes. This confirms the fact of right ovary being more active than the left one. Moreover, the biometry of ovary and tubular parts of genitalia in Local×Holstein Friesian and Local×Jersey cows are suggestive for selection of crossbred dairy cows to get maximum benefits from crossbreeding in respect of productive and reproductive performance and also for genetic improvement.