Abstract: Indonesia has several breeds of goats as the source of genetic. This study aimed to characterize genetic of Indonesian goats. The phylogenetic relationship among and genetic variability within eight native Indonesian goats were analyzed using mtDNA D-loop region sequences. The results show that the length of complete sequences mtDNA D-loop in Indonesian goats are1212 bp. There are 68 polymorphic sites and 21 different haplotypes. Haplotype diversity and nucleotide diversity based on mtDNA D-loop in eight breeds of Indonesian goats are 0.5000-1.000 and 0.248-1.1595%, respectively. The value of genetic distance between groups of native Indonesian goats ranged from 0-0.011. Indonesia goat genetic distance value against wild goat Capra aegagrus (AB004082) is in the range 0.0038-0.045 while against Capra falconeri (AB044306) is 0.087-0.095. The results indicate that the genetic diversity of Indonesian goats is not abundant. Indonesian goats originated from one maternal. Maternal origin of Indonesian goats based on mtDNA D-loop is lineage B.