Abstract: Effect of varying doses of Ovatide on the breeding performance of Clarias gariepinus in Sokoto, North-western Nigeria was investigated. The females weighed between 330 to 1078.50 g while the males weighed 317 to 542.40 g. Eight gravid brood stocks were injected intramuscularly at different dose levels of 0.10 (treatment I), 0.15 (treatment II), 0.20 (treatment III) and 0.25 (treatment IV) mL kg-1 b.wt. (Bw), at a water temperature of 30°C to assess their breeding performance. The experiment was replicated in a completely randomized design. The results showed that the size of eggs did not vary among the samples based on all the treatments. The female of treatment IV had the highest spawning fecundity (149,796), weight (228 g) and percentage of eggs stripped (21.14%). Treatment III had the highest percentage of fertilized eggs (95.45%) which was significantly (p<0.05) higher than in the other treatments. Treatment II had the highest hatchability (65.28%), number of hatchlings (3,017) and total weight of hatchlings (12.07 g). It was concluded that C. gariepinus of about 400 g can successfully be induced to ovulate using 0.15 mL kg-1 b.wt. in Sokoto, Nigeria which was lower than the manufacturer’s recommended dosage of 0.20 mL kg-1 b.wt.