Abstract: In this study, we describe the insertion/deletion (indel) polymorphism of the THRSP gene evaluated using 1% agarose gel and 8% polyacrylamide gel electrophoresis in a sample of (17 breeds of chicken including 3 foreign breeds and 14 chinese native breeds). For the THRSPα gene indel polymorphism result, allele frequencies varied, between chinese native chicken and a foreign chicken. A high frequency of allele B (0.91) and a low frequency of allele A (0.09) were observed in Leghorn chicken, which has been selected for laying performance. Based on variation in allele frequencies among populations, either the low frequency of allele A or the high frequency of allele B could be linked to fatness trait of the chicken. In conclusion, THRSPα gene might be used as a molecular marker in high quality broiler assistant selection breeding program.