Abstract: Background and Objective: Yield has always been the major constraint for most crops, especially for rice. Grains, being the end product of rice plant can only be acknowledged during the grain filling period, the last stage in the life cycle of rice plants. Comparison of biochemical properties with its yield during the early stage, can act as an early selection criteria for high yielding traits. This study was conducted to evaluate the effectiveness of Rubisco activity, chlorophyll content, starch content, soluble protein content and dry weight measurement of leaves seedlings on 12 recommended local rice cultivars in comparison with its yield potential. Materials and Methods: The experiment was performed in a completely randomized block design. A total of 5 plants per cultivar were grown and 3 plants were randomly picked for biochemical analyses. The collected results were analyzed statistically using the analysis variance (one-way ANOVA) and Tukey’s test. Results: The data obtained revealed that Rubisco activity, protein content and dry weight in leaves seedlings showed no correlation with yield with p>0.05. In contrast, both chlorophyll content and starch content showed a significant difference (p<0.05) that indicated variation among the cultivars. Chlorophyll content was found negatively correlated with yield in most of the studied cultivars. Conclusion: The potential of early detection using biochemical assessments in leaves seedlings of rice cultivars are in relation with its yield.