Abstract: In order to investigate the effect of salt stress on chlorophyll content and fluorescence, sodium (Na+) and potassium (K+) ions content of rape (Brassica napus L.) plants, ten genotypes were subjected to salinity levels (control [2.5], 6, 10, 14 and 18 dS m-1) for 30 days in hydroponics. Salt treatments were imposed to genotypes in root establishment stage (4 leaves). Results showed that quantum yield of photosystem II from light adapted (ΦPSII) and dark-adapted leaf (Fv/Fm), photochemical quenching (qP) and minimal fluorescence from dark-adapted leaf (Fo) were affected by salinity. Genotypes MHA4921 and Hyola 401 had highest shoot dry weight at the two higher salt treatments (14 and 18 dS m-1) and resulted the most tolerant to salinity among the tested genotypes. Chlorophyll (chl) fluorescence attributes was generally affected by salinity stress, except in the two salt tolerant genotypes and thus could be used as a tool for screening for salinity tolerance. Chlorophyll content (SPAD units) changed significantly in all genotypes, except in salt tolerant ones. Shoots Na+ content increased, by increasing salinity levels, but in MHA4921 this increase was higher than the other genotypes and may be relation to decline in the osmotic potential of cellular contents. Rape ability to accumulate sodium in response to salinity is one of the major criteria of salt tolerance. K+ content in shoots, at the different levels of salinity in MHA4921 and Hyola 401 were higher than the other genotypes.