Abstract: Background and Objective: Despite the agricultural importance of glyphosate, there are concerns bordering on their effects on the environment and man. This study was, therefore, designed to analyze the adverse effects of glyphosate on sperm profile and testicular architecture of Wistar rat. Materials and Methods: Twenty four mature male Wistar rats were divided into 4 groups (A-D) of 6 rats each in a completely randomized design. Group A served as the control, groups B, C and D received 100, 200 and 300 mg kg–1 b.wt., of glyphosate, respectively. Treatments were administered via oral gavage for 2 months after which the animals were sacrificed under chloroform anesthesia 24 h after the last dose. Sperm and testes samples were collected for examination and analyses. Data obtained were analyzed by using one way Analysis of Variance (ANOVA). Results: The results revealed a significant (p<0.05) and dose dependent decrease in testes weight, mean sperm motility, viability and count and an increase in sperm head abnormality of rats administered glyphosate treatment compared to the control. Testes photomicrograph of glyphosate treated rats showed mild to severe degeneration of sertoli cells, eroded interstitial cells and atrophy of some spermatogonia cells which was indications of testicular toxicity. Conclusion: Therefore, glyphosate adversely affected sperm quality, sperm quantity and destroyed the cellular architecture of the testes of Wistar rats. These findings indicate that glyphosate could induce infertility in males.