Abstract: Background and Objective: Waste management is becoming a vital environmental issue now a day. Present study attempted to figure out harmful microorganisms in both treated and untreated pharmaceutical wastes, the drug-resistance pattern of the microbial isolates from the selected wastes, the anti-bacterial activity of the waste samples and the increased bacterial resistance against different antimicrobial agents possibly triggered by the biofilm formation. Materials and Methods: Isolation and identification of pathogenic microorganisms was accomplished through conventional and cultural methods and the disc diffusion (Kirby-Bauer test) method was intruded for antibiogram and antimicrobial activity measurement. Results: The total viable bacteria and fungi was estimated up to 106 and 103 CFU mL–1, respectively in the untreated wastes while the microbial load elevated up to 108 and 106 CFU mL–1, respectively in the treated waste samples. While the bacterial load was noticed to be increased in all the treated wastes up to a quantity of 105 CFU mL–1, only four antibiotics out of twelve were found to be effective against the isolates. Interestingly, after forming biofilms, all the isolates showed resistance against the drugs tested. The untreated wastes unveiled their massive anti-bacterial traits against the soil bacteria isolated from the surroundings of pharmaceuticals. Conclusion: The research projected on the continuous exposure of the environment to a range of antibiotics and the drug-resistance genes within the environmentally available microbial consortia posing dreadful impact on public health.