Abstract: Background and Objective: Genetic diversity and variability in the population are pre-requisite for the crop improvement programme. Collection and maintenance of genetic diversity is a fundamental component in long-term management strategies for genetic improvement of silkworm. The silkworms mutant were evaluated for morphological characters and rearing traits during two crop seasons i.e., winter and summer to analyze the performance and to identify better mutant accessions. Materials and Methods: Total 23 mutant silkworm accessions were considered for the study, the important morphological characters during egg, larva and cocoon stages were recorded and compared with the catalogue data. Total 12 important silkworm quantitative traits were studied and analysed using multi trait analysis package to identify better accessions. Results: Significant amount of variations were observed among different accessions for different traits. Among 23 mutant silkworms 08 were qualified in the cumulative Evaluation Index (EI) (>50). The cluster analysis showed heterogeneity among the silkworm accessions based on the grouping. The Principal Component Analysis (PCA) indicated the grouping of 06 mutants along with commercially important silkworm races Multivoltine Pure Mysore (PM) and bivoltine (CSR-2). Conclusion: Since these genotypes were considered most suitable for basic genetic studies rather its usefulness in silk production. The correlation studies using PCA revealed that some of the mutant silkworms conserved in the germplasm showed on par with commercially important silkworm races and can be used to explore the combining ability studies for further commercial exploitation.