Abstract: Background and Objective: Canola (Brassica napus L.) is an important source of vegetable oil and is the second largest oil seed crop after soybean. Many analytical techniques have been reported for the identification and quantification of proteins. In this study, the main objective was to evaluate the most effective protein isolation method from canola seeds. Materials and Methods: Four different winter canola types from Brassica napus subsp. oleifera seeds were cultured in Turkey. Proteins were isolated using four methods from these types of canola and were analyzed by SDS-PAGE method. Phenylalanine Ammonia-Lyase (PAL) enzyme activities of canola types were measured. Besides, high performance liquid chromatography (HPLC) chromatographic method was performed in order to obtain the cinnamic acid amount in the methanol extracts of the canola seeds. Results: The TCA method yielded the highest protein contents among four protein isolation methods. These methods showed that Orkan type contains higher protein. The highest PAL enzyme activity was found at Eurol and California types. According to HPLC results, the highest amount of cinnamic acid was determined in Eurol type. Conclusion: Protein isolation and identification from canola types containing such active specific proteins is of great importance both economically and scientifically. For protein isolation from canola seeds, TCA method yielded was the highest protein contents.