Abstract: Background and Objective: The indeno quinoxaline pyrrolothiazole derivative crystal of ethyl 6'-cyano-7'-(p-tolyl)-1',5a,6’,7',7a',9a-hexa hydro-3'H-spiro [indeno [1,2-b]quinoxaline-11,5'-pyrrolo[1,2-c] thiazole]-6'-carboxylate (ECPTHSIPTC) was crystallized from slow evaporation method at room temperature. The grown crystal was characterized by the FT-IR, FT-Raman and UV-Visible spectroscopy techniques. The complete vibrational spectra of various functional groups wavenumber assignment for title crystal were interpreted in the present work. Materials and Methods: Ethyl cyano acetate, thioproline, benzaldehyde, indeno quinoxaline, ethyl acetate and chloroform were the raw materials used to synthesis the title compound. The synthesized compound was recrystallized from chloroform solution by the slow evaporation method. Results: FT-IR and FT-Raman spectrometers were used to record the vibrational spectra of title crystal at room temperature in the wavenumber range 4000- 400 cm–1. The optical property was performed by double beam spectrophotometer in the wavelength range 200−1100 nm. Conclusion: The both spectroscopy studies confirmed the various functional groups present in the title compound. The UV-Visible spectroscopy study showed that the grown crystal has high transparency in the entire visible region and the optical band gap was found as 3.25 eV. The ECPTHSIPTC crystal was taking into account to study and investigated in this present work due to the wide-ranging applications in biological and pharmaceutical fields.