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Trends in Molecular Sciences
  Year: 2011 | Volume: 3 | Issue: 1 | Page No.: 25-31
DOI: 10.3923/tms.2011.25.31
 
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DNA Molecular Analysis between Lethal Yellowing Disease and Non-lethal Yellowing Disease of Coconut Palms (Cocos nucifera L.) in Nigeria

I.B. Omamor, E.I. Eziashi, O.A. Asemota, C.I. Aisagbonhi and L.A. Ogunkanmi

Abstract:
The aim of this study was to establish phyllogenetic similarity between lethal yellowing susceptible and apparently healthy Coconut palm varieties in Nigeria. Lethal Yellowing Disease (LYD) of coconut palm is caused by phytoplasma, the organism is not detectable in culture media. It has practically wiped off great majority of the palms in South East, South-South and is now extending towards the South West. Crownless coconut palms are a common sight in these areas. Despite the wide geographical occurrence, no report is available on phyllogenetic similarity between LYD and non-LYD palms. In this study, Random Amplified Polymorphic DNA (RAPD) was used to detect the phyllogenetic similarity of 16 coconut varieties representing 4 species. A total of 30 polymorphic RAPD bands were detected. Similarity Coefficient (SC) between varieties ranged from 0.75 to 1.00 showing high level of similarity. Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendrogram clearly separated the LYD and non-LYD varieties. At SC value of 0.75, RAPD markers separated the hybrid and Malayan dwarf green from Malayan dwarf yellow and Malayan dwarf red varieties. At the value of 0.79, LYD and non-LYD varieties were grouped into four major clusters. First and second clusters distinguished themselves separately while the third and fourth, clustered together. Result indicated that hybrid (5S) and Malayan dwarf green (4S) varieties exhibited better non-LYD with Distant Coefficient (DC) from LYD infected varieties screened. This result could be exploited in breeding program.
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How to cite this article:

I.B. Omamor, E.I. Eziashi, O.A. Asemota, C.I. Aisagbonhi and L.A. Ogunkanmi, 2011. DNA Molecular Analysis between Lethal Yellowing Disease and Non-lethal Yellowing Disease of Coconut Palms (Cocos nucifera L.) in Nigeria. Trends in Molecular Sciences, 3: 25-31.

DOI: 10.3923/tms.2011.25.31

URL: https://scialert.net/abstract/?doi=tms.2011.25.31

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