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Trends in Medical Research
  Year: 2014 | Volume: 9 | Issue: 1 | Page No.: 17-32
DOI: 10.3923/tmr.2014.17.32
Immunohistochemical and Ultra Structural Study of Mammary Myoepithelial Cells in Pregnant and Lactating Rats
Nesreen Moustafa Omar

Abstract:
Myoepithelial cells are highly specialized cells which express cytoskeletal and contractile proteins such as α-smooth muscle actin (α-SMA). This study was performed in order to determine the immunohistochemical and ultra structural features of mammary myoepithelial cells during pregnancy and lactation. Fifteen adult female albino rats assigned into 3 groups; group I (virgin), group II (pregnant) and group III (lactating); were used in this study. Specimens from the mammary gland were obtained and processed for light and electron microscopy. Paraffin sections of the mammary gland were prepared and stained with H and E stain and immunohistochemical stain using anti α-SMA antibody. In the resting mammary gland, the myoepithelial cells (ME) formed a single continuous layer around the mammary ducts. During pregnancy, ME cells were more developed forming multilayered sheaths around the ducts which may protrude to the stroma. Around the acini, myoepithelial cells were branched forming a discontinuous layer around the acinar cells. During lactation, ME cell were stretched, faintly stained, discontinuous and acquired some ultra structural features of smooth muscles. It could be concluded that mammary myoepithelial cells undergo morphological alterations which correlate with the physiological requirements during pregnancy and lactation.
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How to cite this article:

Nesreen Moustafa Omar , 2014. Immunohistochemical and Ultra Structural Study of Mammary Myoepithelial Cells in Pregnant and Lactating Rats. Trends in Medical Research, 9: 17-32.

DOI: 10.3923/tmr.2014.17.32

URL: https://scialert.net/abstract/?doi=tmr.2014.17.32

 
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