The increased risk of morbidity and mortality in obesity as a result of infections is an emerging health concern which suggests that obesity may affect immune competence. Thus aim of this study was to assess circulating immune cell parameters including T lymphocyte subsets and Natural killer (NK) cells in obese subjects and their relations to metabolic factors. Thirty obese and 20 lean subjects were included in the study. Recorded data involved BMI, Waist Circumference (WC), blood pressure, HOMA-IR, White Blood Cell (WBC) count, Absolute Lymphocyte Count (ALC),CD4 and CD8 T cell count and frequency and natural killer cell frequency (CD56%). CD4, CD8 and CD56 expressions were measured by flow cytometry. Compared with control, obese group had significantly higher CD4%, CD4 count, WBC count and ALC, and significantly lower CD8%, CD8 count and CD56%. In obese subjects, both CD4% and CD4 count had significant positive correlation with BMI (p = 0.005, p<0.001, respectively) and WC (p = 0.014, p = 0.01, respectively). CD4 count had significant positive correlation with systolic blood pressure (p = 0.015), HOMA-IR (p = 0.021) and triglycerides (P=0.02). CD8 count in obese subjects had significant negative correlation with BMI (p = 0.03), WC (p = 0.018), HOMA-IR (p = 0.035) and triglycerides (p = 0.025), while CD56% had significant negative correlation with BMI (p = 0.025), HOMA-IR (p = 0.04) and triglycerides (p = 0.029). Obesity is associated with alterations in circulating immune cells in the form of increased WBC, total lymphocyte and CD4 levels with decreased CD8 and CD56 levels, such alterations are related to obesity measures and metabolic factors, so immune alterations may identify obese at risk of infection and metabolic disease who should be targeted for weight loss strategies. Further studies are needed to determine the role of weight loss in the improvement of immune function in obese individuals.