Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Trends in Medical Research
  Year: 2011 | Volume: 6 | Issue: 2 | Page No.: 116-123
DOI: 10.3923/tmr.2011.116.123
Detection of Biofilm Formation in Staphylococcus aureus. Does it have a role in Treatment of MRSA Infections?
Fatima Khan, Indu Shukla, Meher Rizvi, Tariq Mansoor and S.C. Sharma

Abstract:
Staphylococcus aureus is a causative agent of many types of diseases throughout the world. Staphylococcal infections are of particular concern because of the causative bacteria offering resistance to a wide range of commonly used antibiotics. The formation of biofilm is the hallmark characteristic of S. aureus infection. Biofilms constitute reservoir of pathogens and are associated with resistance to antimicrobial agents and chronic infections. In this study 262 clinical strains of S. aureus were screened by tissue culture plate method, tube method and congo red agar method for biofilm formation. Antimicrobial sensitivity testing of these strains was done by Kirby bauer disc diffusion method. Tissue culture plate method detected 38 (14.51%) isolates as strong biofilm producers, 132 (50.38%) as moderate biofilm producers and 92 (35.11%) strains as nonproducers of biofilm. The congo red agar method had a low sensitivity and specificity of 67.65 and 89.13%. The tube method correlated well with tissue culture plate method with a sensitivity and specificity of 99.40 and 95.78% but the interpretation are observer dependent. Biofilm production was higher in methicillin resistant strains as compared to the methicillin sensitive strains of S. aureus. Biofilm producers were found to be more resistant to almost all the groups of antibiotics.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Screening of Microbes for Their Metal, Antibiotic Resistance and Plant Growth Promoting Activity
  •    Biosynthesis of Hygromycin-B Antibiotic by Streptomyces crystallinus AZ151 Isolated from Assuit, Egypt
  •    Molecular Fingerprinting of Methicillin-Resistant Staphylococcus aureus Isolates in Hospital Staff and Patients
  •    Antibacterial Activity of Certain Iranian Medicinal Plants Against Methicillin-Resistant and Sensitive Staphylococcus aureus
  •    Evaluating the Antibacterial Activity of Elephantopus scaber Extracts on Clinical Isolates of β-lactamase Producing Methicillin Resistant Staphylococcus aureus from UTI Patients
  •    In vitro Activity of Some Antimicrobial Agents against Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Khartoum, Sudan
How to cite this article:

Fatima Khan, Indu Shukla, Meher Rizvi, Tariq Mansoor and S.C. Sharma, 2011. Detection of Biofilm Formation in Staphylococcus aureus. Does it have a role in Treatment of MRSA Infections?. Trends in Medical Research, 6: 116-123.

DOI: 10.3923/tmr.2011.116.123

URL: https://scialert.net/abstract/?doi=tmr.2011.116.123

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 

       

       

Curve Bottom