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Trends in Medical Research

Year: 2007 | Volume: 2 | Issue: 4 | Page No.: 167-175
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Authors

Somasundaram Thirugnanasambandan Somasundaram

Pandian Vijayabaskar

Keywords

Glycosaminoglycan (GAG) of heparin family was localized in the tissues of bivalve Katelysia opima. Different histological staining procedures like alcian blue, aldehyde fuchsin and periodic acid-Schiff’s reagent (PAS) were followed to test the presence of GAG in various tissues of this bivalve mollusk. The alcian blue with two different pH and combination of alcian blue-aldehyde fuchsin staining solutions indicated the presence of sulfated polysaccharide. The GAG was found in the tissues like gill, palp, foot, mantle and adductor muscle in an order of decreasing pattern. Crude GAG was isolated from these tissues by cetyl pyridinium chloride (CPC) precipitation method and the obtained product was tested for its anticoagulant property along with standard heparin. The isolated GAG was also compared with standard heparin through FT-IR to find its relevant functional groups. The bivalve GAG was subjected to fractionation for further purification and its chemical components were analyzed. The subsequent comparison with the commercial heparin showed that K. opima could be a potent source of heparin for commercial use.
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Somasundaram Thirugnanasambandan Somasundaram and Pandian Vijayabaskar, 2007. Histological and Analytical Evaluation of Glycosaminoglycan from the Clam Katelysia opima. Trends in Medical Research, 2: 167-175.

URL: https://scialert.net/abstract/?doi=tmr.2007.167.175

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