Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Trends in Bioinformatics
  Year: 2014 | Volume: 7 | Issue: 1 | Page No.: 19-26
DOI: 10.3923/tb.2014.19.26
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
In silico Analysis of Essential Tricarboxylic Acid Cycle Enzymes from Biofilm-forming Bacteria
M.F.Z.R. Yahya, U.M.A. Hamid, M.Y. Norfatimah and R. Kambol

Abstract:
The Tricarboxylic Acid Cycle (TCA) cycle is the central point in the metabolism of living organisms and is important for the survival of infectious biofilms. The inhibition of this vital point could be a promising strategy for the control of infectious biofilms. Therefore, this study was carried out to identify the potential drug targets from the TCA cycle of several Biofilm-Forming Bacteria (BFB) and to identify the available small molecule drugs against the TCA cycle enzymes. Based on the in silico substractive genomic approach, citrate lyase subunit alpha/citrate CoA-transferase [EC: 4.1.3.6], succinate dehydrogenase iron-sulfur subunit (EC: 1.3.99.1) and 2-oxoglutarate ferredoxin oxidoreductase subunit delta [EC: 1.2.7.3] were found to be essential and exclusively present in the BFB. Further in silico analyses showed that most of them are chemically regulated by myristoylation, phosphorylation, glycosylation and amidation. Based on the sequence search against DrugBank database, the potential small molecule drugs for biofilm treatment are 2-[1-methylhexyl]-4, 6-dinitrophenol, Atpenin A5 and Ubiquinone-2 which all target the succinate dehydrogenase enzyme of BFB. This study demonstrates the rapid identification of potential drug targets and small molecule drugs which could be useful in biofilm control strategies.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

M.F.Z.R. Yahya, U.M.A. Hamid, M.Y. Norfatimah and R. Kambol, 2014. In silico Analysis of Essential Tricarboxylic Acid Cycle Enzymes from Biofilm-forming Bacteria. Trends in Bioinformatics, 7: 19-26.

DOI: 10.3923/tb.2014.19.26

URL: https://scialert.net/abstract/?doi=tb.2014.19.26

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 

       

       

Curve Bottom