Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
Trends in Applied Sciences Research
  Year: 2010 | Volume: 5 | Issue: 3 | Page No.: 188-196
DOI: 10.3923/tasr.2010.188.196
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Identification and Differentiation of Orseolia Species in Nigeria as Revealed by SCAR-PCR Analysis

Francis E. Nwilene, Amos Onasanya, Tolulope A. Agunbiade, Mark N. Ukwungwu, Yacouba Sere, Ivan Ingelbrecht, Abou Togola, Dona Dakouo, Ba Malick, Souleymane Nacro, Abdoulaye Hamadoun, Noe Woin and James Charles

Abstract:
Identification and differentiation of Orseolia species in Nigeria was carried out using SCAR-PCR analysis. Twenty-three insects from 9 localities in Nigeria and three reference insects (Orseolia bonzii, Orseolia nwanzei and Orseolia oryzivora) were analyzed. Out of the 60 SCAR primers screened, only four produced clear amplified DNA fragments at annealing temperature of 55°C that differentiated all the 26 Orseolia species. Cluster analysis revealed two major insect genotypes (OSG-1 and OSG-2). The OSG-1 was further divided into two subgroups (OSG-1a and OSG-1b). Eleven insects were genotyped as OSG-1a, 14 as OSG-1b and one as OSG-2. Only NG1 and NG2 were identical among the insects of OSG-1a genotype. OSG-1b genotype produced two different groups of identical insects. While O. bonzii and O. oryzivora were genotyped as OSG-1b along with other twelve insects, only O. nwanzei was genotyped as OSG-2. OSG-1b genotype constitutes about 54% O. bonzii and O. oryzivora in Nigeria, OSG-2 genotype represents 4% of O. nwanzei in Nigeria while OSG-1a genotype covers 42% of yet unknown Orseolia species in Nigeria. This information would strongly assist breeding programmes aiming at effective development of cultivars with durable resistance to African rice gall midge (AfRGM) in Nigeria.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Francis E. Nwilene, Amos Onasanya, Tolulope A. Agunbiade, Mark N. Ukwungwu, Yacouba Sere, Ivan Ingelbrecht, Abou Togola, Dona Dakouo, Ba Malick, Souleymane Nacro, Abdoulaye Hamadoun, Noe Woin and James Charles, 2010. Identification and Differentiation of Orseolia Species in Nigeria as Revealed by SCAR-PCR Analysis. Trends in Applied Sciences Research, 5: 188-196.

DOI: 10.3923/tasr.2010.188.196

URL: https://scialert.net/abstract/?doi=tasr.2010.188.196

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

Curve Bottom