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Trends in Applied Sciences Research
  Year: 2007 | Volume: 2 | Issue: 5 | Page No.: 419-425
DOI: 10.3923/tasr.2007.419.425
 
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Immobilization of Aspergillus niger Pectinase on Polyacrylonitrile Copolymer Membrane
Ginka Delcheva, Ivan Pishtiyski , Georgi Dobrev and Svetla Krusteva

Abstract:
Aspergillus niger pectinase was successively immobilized on polyacrylonitrile copolymer membrane. The optimal duration of immobilization process and optimal enzyme concentration of 1% (w/v) at pH 3.0 were determined. Immobilized enzyme showed a shift in pH optimum which was at pH 5.0 for the enzyme immobilized on not activated membrane and at pH 5.5 for the enzyme immobilized on membrane activated with glutaraldehyde. Both immobilized enzymes exhibited maximum activity at 60°C. Free pectinase exhibited higher stability than that of the immobilized enzyme at 30, 40 and 50°C. In order to examine the reusability of immobilized pectinase 22 consecutive batch cycles were performed.
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How to cite this article:

Ginka Delcheva, Ivan Pishtiyski , Georgi Dobrev and Svetla Krusteva , 2007. Immobilization of Aspergillus niger Pectinase on Polyacrylonitrile Copolymer Membrane. Trends in Applied Sciences Research, 2: 419-425.

DOI: 10.3923/tasr.2007.419.425

URL: https://scialert.net/abstract/?doi=tasr.2007.419.425

 
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