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Trends in Applied Sciences Research
  Year: 2007 | Volume: 2 | Issue: 3 | Page No.: 201-210
DOI: 10.3923/tasr.2007.201.210
Determination of a Minimal DNA Sequence of the Internal Transcribed Spacer Region for the in silico Identification of Botryosphaeria sp
L.A. Vilas-Boas , M.A. Coronado , G.T. Vilas-Boas , R.F.H. Dekker , A.M. Barbosa and J.E. Garcia

Abstract:
Botryosphaeria species are plurivorous phytopathogenic fungi colonizing a wide range of host plants of agricultural, forestry, ecological and economic importance. Species identification in the Botryosphaeria genus is based mainly on morphological characteristics of the anamorphs in combination with sequence analysis from the Internal Transcribed Spacer (ITS) region. The purpose of this study was to determine a minimal DNA sequence of the ITS region which could be applied for the identification of Botryosphaeria species. A total of 23 entries from the ITS sequences of 13 Botryosphaeria species and several fragment lengths obtained from these entries, were compared with those already deposited in public databases using the BLASTn search tool. The secure identification of 11 of the 13 studied species was possible from the start of the ITS1 and the end of the ITS2 region using fragments up to 200 bp. These results were similar to those obtained with the complete ITS1/5.8S/ITS2 region. Phylogenetic trees based upon the neighbor-joining method showed similar topology when generated by complete ITS sequence and compared with those obtained with 200 bp sequences. The results in this study showed that for identification of the greater number of the Botryosphaeria species, a simple sequence read with 200 bp obtained from the beginning of the ITS1 region, or from the end of the ITS2 region should be sufficient. This procedure makes the identification process more rapid and easier and reduces the assembly efforts.
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How to cite this article:

L.A. Vilas-Boas , M.A. Coronado , G.T. Vilas-Boas , R.F.H. Dekker , A.M. Barbosa and J.E. Garcia , 2007. Determination of a Minimal DNA Sequence of the Internal Transcribed Spacer Region for the in silico Identification of Botryosphaeria sp . Trends in Applied Sciences Research, 2: 201-210.

DOI: 10.3923/tasr.2007.201.210

URL: https://scialert.net/abstract/?doi=tasr.2007.201.210

 
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