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Singapore Journal of Scientific Research
  Year: 2021 | Volume: 11 | Issue: 1 | Page No.: 38-45
DOI: 10.3923/sjsres.2021.38.45
 
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In vitro and in vivo Antioxidant Activity of Crude Methanolic Leaves Extract of Acacia nilotica (Linn.)

Abubakar Abdulhamid , Amar Mohamed Ismail, Ibrahim Sani and Abdullahi Sulaiman

Abstract:
Background and Objective: Many synthetic antioxidants were reported to have several side effects, such as the risk of liver damage and carcinogenesis in laboratory animals. This has led to the search for more effective, less toxic and cost-effective natural antioxidants, particularly from plants. This research aimed to evaluate the in vitro and in vivo antioxidant potentials of crude methanolic leaves extract of Acacia nilotica. Materials and Methods: The extract was evaluated for its antioxidant compounds, in vitro and in vivo antioxidant activities. The levels of flavonoids, total phenolic compounds, ascorbic acid and α-tocopherol were determined. Results: The DDPH radical scavenging activity and the ferric reducing the power of the extract were found to be high and comparable to that of standard ascorbic acid. The result of the in vivo antioxidant activity showed that CCl4 treatment caused a significant (р<0.05) decrease in the plasma GSH level compared to that of the normal group treatment. The 150, 300 and 600 mg kg1 b.wt. of extracts pre-treatments for 7 days significantly (p<0.05) enhanced the level of GSH when compared to the toxic (CCl4) control groups, but lower when compared to positive (ascorbic acid) and normal control groups. Also, elevated MDA levels were observed for the CCl4 treated group (toxic control) than for other control samples, with the ascorbic acid pre-treatment showing the least MDA levels. Test samples (150-600 mg kg1 b.wt.) showed a significantly (p<0.05) lower MDA levels in the liver tissues compared to the CCl4 control group. Conclusion: The results obtained in the present study indicate that the leaves of A. nilotica are a potential source of natural antioxidants, which may provide leads in the ongoing search for natural antioxidants from plants.
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How to cite this article:

Abubakar Abdulhamid, Amar Mohamed Ismail, Ibrahim Sani and Abdullahi Sulaiman, 2021. In vitro and in vivo Antioxidant Activity of Crude Methanolic Leaves Extract of Acacia nilotica (Linn.). Singapore Journal of Scientific Research, 11: 38-45.

DOI: 10.3923/sjsres.2021.38.45

URL: https://scialert.net/abstract/?doi=sjsres.2021.38.45

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