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Singapore Journal of Scientific Research
  Year: 2012 | Volume: 2 | Issue: 1 | Page No.: 25-32
DOI: 10.3923/sjsres.2012.25.32
Performance Analysis of Multiplex-PCR based Detection of Salmonella sp. and Salmonella Typhimurium in Chicken Egg Samples
Monzur Morshed Ahmed, Md. Siddique Hossain, Khandaker Rayhan Mahbub, Himel Nahreen Khaleque, Zakir Hossain, Md. Fakruddin, Abhijit Chowdhury, Md. Nur Hossain and Md. Zobaidul Alam

Abstract:
Polymerase Chain Reaction (PCR) based molecular detection techniques of food pathogens are taking place of conventional media based biochemical methods due to more quick and reliable performances. Detection of Salmonella sp. from food matrixes requires several steps, culture media and at least four consecutive days of incubation period, therefore we designed primer based (invA and fliC genes) multiplex-PCR analysis for detection of Salmonella sp. and Salmonella Typhimurium from chicken egg samples of local market in Dhaka city. The prevalence of Salmonella spp. and Salmonella Typhimurium were analyzed by standard biochemical detection method as well as multiplex-PCR technique from a total of 200 egg samples. The primers were selected for the presence of invA and fliC genes of Salmonella sp. and Salmonella Typhimurium, respectively. Twenty four samples out of 200 egg samples were found positive for Salmonella sp. through standard culture technique, on the other hand among all the biochemically positive samples, only 16 samples (66%) offered excellent response in multiplex-PCR assay. The results indicate that the present multiplex-PCR technique requires further optimization for getting higher response for detection of Salmonella sp. and Salmonella Typhimurium from egg samples.
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How to cite this article:

Monzur Morshed Ahmed, Md. Siddique Hossain, Khandaker Rayhan Mahbub, Himel Nahreen Khaleque, Zakir Hossain, Md. Fakruddin, Abhijit Chowdhury, Md. Nur Hossain and Md. Zobaidul Alam, 2012. Performance Analysis of Multiplex-PCR based Detection of Salmonella sp. and Salmonella Typhimurium in Chicken Egg Samples. Singapore Journal of Scientific Research, 2: 25-32.

DOI: 10.3923/sjsres.2012.25.32

URL: https://scialert.net/abstract/?doi=sjsres.2012.25.32

 
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