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Singapore Journal of Chemical Biology

Year: 2016 | Volume: 5 | Issue: 1 | Page No.: 1-10
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Authors

F.J. Al-Saffar

F.J. Al-Saffar

Eyhab R.M. Al-Samawy

Keywords

Research Article

Histomorphological and Histochemical Study of the Small Intestine of the Striated Scope Owls (Otus Scors Brucei)

F.J. Al-Saffar F.J. Al-Saffar's LiveDNA and Eyhab R.M. Al-Samawy
Objective: This project aimed to study the histomorphological and histochemical structures of the wall of the small intestine of Striated Scope Owls (Otus Scors brucei) in Iraq. Methodology: To conduct this investigation, 12 healthy owls were collected from local suppliers. Birds were euthanized, dissected and then specimens were processed for histological and histochemical staining techniques. Results: Gross findings showed that the small intestine was consisted of 3 organs (duodenum, jejunum and ileum). Histologically, the small intestine was lined by simple columnar with goblet cells. The muscularis mucosa in the duodenum arranged in one longitudinally set fibers. The submucosa was thick layer of loose connective tissue. Tunica serosa was thin layer of areolar loose connective tissue. In the jejunum, the mucous membrane was thrown into large numerous villi showed blunt apical part and wide basal part with absence of muscularis mucosa. In the ileum, both simple columnar cells and goblet cells were observed and similar to jejunum, muscularis mucosa was absent. Histochemical findings showed that the columnar cells were negatively reacted with the PAS stain, whereas the goblet cells were strongly reacted with this stain. On applying the combined PAS-AB (pH 2.5), the wall showed epithelial cells stained negatively with this stained while the goblet cells gave strong positive reaction (dark blue). The combined PAS-AB (pH 1) showed strong reaction with the goblet cells for their neutral mucopolysaccharides but the columnar epithelium showed poor reaction. Intestinal mucosal lining revealed no response toward mercury bromophenol blue staining, while, the submucosal connective tissue revealed positive reaction for this technique. The columnar cells of small intestine gave the negative reaction with PAS, PAS-AB (pH 2.5) and PAS-AB (pH 1), whereas, the goblet cells were strongly positive reacted. Conclusion: Because the bird is carnivores species, the columnar cells of small intestine gave the negative reaction with PAS, PAS-AB (pH 2.5) and PAS-AB (pH 1), whereas, the goblet cells were strongly positive reacted. Infact, the latter stain is an indicator for sulfated acidic mucin substances which are very important in digestion and absorption and subsequent body growth of the bird.
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How to cite this article

F.J. Al-Saffar and Eyhab R.M. Al-Samawy, 2016. Histomorphological and Histochemical Study of the Small Intestine of the Striated Scope Owls (Otus Scors Brucei). Singapore Journal of Chemical Biology, 5: 1-10.

URL: https://scialert.net/abstract/?doi=sjchbio.2016.1.10

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