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Research Journal of Veterinary Sciences
  Year: 2016 | Volume: 9 | Issue: 1 | Page No.: 19-25
DOI: 10.3923/rjvs.2016.19.25
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Approaching Insights to in vitro Production of Bovine Embryos and the Growth Rate to Blastocyst

A.A. Ezzat , T.I. Hirata and T. Hashizume

Background: Cattle oocytes were collected from live animals by Ovum Pick Up (OPU) or from Slaughter House Ovaries (SHO) by aspiration using needle. The in vitro fertilization (IVF) technique consists of in vitro maturation (IVM) of oocytes, fertilization and in vitro cultures (IVC) of the embryos. Mature oocytes are cultured with sperm for fertilization resulting in zygotes formation. Those zygotes are cultured for cleavage till reach the blastocyst stages. Those embryos are transferred to recipients directly or cryopreserved. Materials and Methods: This study presents the characteristics of OPU-embryo growth from alive Japanese black cattle (n = 635) and those originated from SHO (n = 10,147). The ova were processed for selection, cleavage, maturation and fertilization parallel to handling of good spermatozoa used for fertilization. Our methods were commonly used with slight modifications. Differences between OPU and SHO-originated embryos at each growth rate were tested by statistical significance by chi-square. Results: Cleavage rates by 48 h after the IVF of the oocytes were 76.1 and 81.7% for the OPU and SHO, respectively. However, the embryo growth to the blastocyst by 6-9 days later was 38.6 and 28.5%, respectively. The percentage of blastocysts (day 9) is lower than expected because the development of some embryos is arrested at earlier stages in particular those originated from the SHO. Conclusion: The growth results of an OPU origin ovum and those of a SHO origin ovum are almost the same. The growth rate to the blastocyst of an OPU origin ovum is strongly subject to the influence of a sire and donor cow and its variation is large. This study presents a simple lab technique for cattle IVF and recommends the slaughter house ovaries (SHO)-originated oocytes, not risky and easily applied, than those originated by ovum-picking up (OPU) from alive animals. Future studies are required to improve the oocyte maturation percent into blastocyst.
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How to cite this article:

A.A. Ezzat, T.I. Hirata and T. Hashizume, 2016. Approaching Insights to in vitro Production of Bovine Embryos and the Growth Rate to Blastocyst. Research Journal of Veterinary Sciences, 9: 19-25.

DOI: 10.3923/rjvs.2016.19.25






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