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Research Journal of Seed Science
  Year: 2017 | Volume: 10 | Issue: 1 | Page No.: 38-42
DOI: 10.3923/rjss.2017.38.42
 
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Cryopreservation of Selected Physical Dormant Species with Special Focus on Dormancy Breaking Time

Guorong Wu, Ganesh K. Jaganathan, Danping Song and Baolin Liu

Abstract:
Background: Many species with an impermeable seed coat, i.e., physical dormant (PY) have orthodox seeds that can be stored in liquid nitrogen without viablity loss. However, whether the dormancy should be broken before or after storage remains less clear. Objective: The importance of dormancy breaking time during cryopreservation of PY seeds was examined using three species Dodonaea viscosa (Sapindaceae), Senna auriculata (Fabaceae) and Delonix regia (Fabaceae). Materials and Methods: All three species have impermeable seed coats that was broken by dipping in boiling water for 60 sec to make them permeable. Seeds of all three species were divided into two groups with one group receiving dormancy breaking before storage in liquid nitrogen for 2 years and the other was stored dormant. Results: For D. viscosa, storing dormancy broken seeds in liquid nitrogen resulted in significantly less germination percentage after storage compared with the dormant seeds stored directly in liquid nitrogen and dormancy was broken at the end of storage (p<0.05). Similarly, S. ariculata seeds that received dormancy breaking treatment at the end of storage germinated to a higher percentage but the effect was not significant (p>0.05). On the other hand, there was no significant difference in germination percentage between dormant and non-dormant seeds of D. regia stored in liquid nitrogen (p>0.05), although storing dormant seeds and breaking dormancy at the end of storage resulted in higher germination percentage. Conclusion: The results showed that dormancy breaking time could have different effects determining the success of PY species cryopreservation.
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How to cite this article:

Guorong Wu, Ganesh K. Jaganathan, Danping Song and Baolin Liu, 2017. Cryopreservation of Selected Physical Dormant Species with Special Focus on Dormancy Breaking Time. Research Journal of Seed Science, 10: 38-42.

DOI: 10.3923/rjss.2017.38.42

URL: https://scialert.net/abstract/?doi=rjss.2017.38.42

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