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Research Journal of Phytochemistry
  Year: 2021 | Volume: 15 | Issue: 2 | Page No.: 58-65
 
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Development and Validation of an HPTLC Method for Estimation of Berberine in Berberis aristata

Javed Ahamad , Raad A. Kaskoos, Saima Amin and Showkat R. Mir

Abstract:
Background and Objective: High Performance Thin Layer Chromatographic (HPTLC) is a planner chromatographic technique used for the detection and quantification of natural products. An HPTLC densitometric method was developed and validated for the estimation of berberine in Berberis aristata stem bark accessions from the different sub-Himalayan region in India and Nepal is described. Materials and Methods: Sample preparation involved extraction of the powdered sample with the water-ethanol mixture (30:70 v/v) containing HCl (5%) at 50°C for 6 hrs, followed by filtration and partitioning with equal volumes of chloroform thrice. The organic layer was pooled, dried under reduced pressure and redissolved in methanol for analysis by HPTLC. Ascending development on precoated silica gel G F254 plates was carried using toluene-ethyl acetate-formic acid-methanol (9:9:2:3 v/v) as mobile phase. Detection and quantitation of berberine were performed by densitometry at 350 nm. Results: The HPTLC densitometric method was developed and validated as per ICH guidelines for the estimation of berberine in different accessions. The developed method gave well resolved, compact bands of berberine at RF 0.52±0.11. The berberine content among the accessions from India was highest for Tamil Nadu (12.08±1.16%) followed by Jammu and Kashmir (7.28±0.44%), Uttarakhand (6.98±0.31%) and Himachal Pradesh (5.34±0.23%). The specimens from Nepal contained 8.44±0.73% of berberine. Conclusion: The developed method was found precise, robust and accurate and was successfully used for the detection and quantification of berberine in different accessions.
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How to cite this article:

Javed Ahamad, Raad A. Kaskoos, Saima Amin and Showkat R. Mir, 2021. Development and Validation of an HPTLC Method for Estimation of Berberine in Berberis aristata. Research Journal of Phytochemistry, 15: 58-65.

URL: https://scialert.net/abstract/?doi=rjphyto.2021.58.65

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