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Research Journal of Phytochemistry
  Year: 2017 | Volume: 11 | Issue: 1 | Page No.: 1-10
 
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Ethanolic Extract of Canthium coromandelicum Leaves Exhibits Antioxidant, Anti-inflammatory and Apoptotic Activity in DEN-induced Hepatocellular Carcinoma

Thirupathi Anand, Ayu Muthia and Shanmugavadivelu Chandra Mohan

Abstract:
Background and Objective: Canthium coromandelicum is a traditional medicinal plant used in various regions of India for treating many diseases. However, its anticancer effects on hepatocellular carcinoma (HCC) have not yet been studied. The aim of this study was to investigate the chemopreventive action and the possible mechanisms of ethanolic extract of Canthium coromandelicum leaves (CEE) against diethylnitrosamine (DEN)-induced liver cancer in rats. Antioxidant, anti-inflammatory and apoptotic properties of CEE were studied by analyzing various proteins. Materials and Methods: Rats pretreated with leaves of CEE intragastrically at the dose of (500 mg kg–1 b.wt.) for 1 week before the administration of DEN and continued till the end of the experiment (i.e., 16 weeks). Analysis of serum HC-related markers, oxidative stress markers, inflammatory markers, DNA-fragmentation assay, protein and gene analysis of apopotic markers were done. Results: Canthium coromandelicum restored the normal liver function by normalizing the serum markers like AST, ALT, ALP and αFP. The extract significantly reduced oxidative markers like MDA and total ROS and increased antioxidant markers like SOD, CAT, GSH and GPx. Proinflammatory mediators like TNF-α and iNOS protein levels were significantly reduced by Canthium coromandelicum. It also inhibited NF-κB activation by reducing phosphor-IκBα and increased cleavage of caspase-3 as well as DNA damage. Conclusion: The present study concludes that CEE exerts its hepatoprotective effect against HCC through induction of apoptosis via modulating oxidative damage and suppressing inflammatory response.
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How to cite this article:

Thirupathi Anand, Ayu Muthia and Shanmugavadivelu Chandra Mohan, 2017. Ethanolic Extract of Canthium coromandelicum Leaves Exhibits Antioxidant, Anti-inflammatory and Apoptotic Activity in DEN-induced Hepatocellular Carcinoma. Research Journal of Phytochemistry, 11: 1-10.

URL: https://scialert.net/abstract/?doi=rjphyto.2017.1.10

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