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Chemical Constituents and Anti-ulcer Activity of Propolis from the North-West Region of Cameroon |
Tamfu Alfred Ngenge,
Domgnim Mokam Elisabeth Carol,
Talla Emmanuel,
Tan Paul Vernyuy,
Mbafor Tanyi Joseph,
Milena Popova and Vassya Bankova  |
Abstract:
Three extracts of propolis harvested from Nkambe, North-West region of Cameroon were characterized by GC-MS analysis and their gastric cytoprotective, antisecretory and antioxidant properties evaluated using experimentally-induced gastric ulcers in rats. The propolis extracts were rich in phytoconstituents occurring as sugars, triterpenes and a mixture (fatty acids+triterpenes+alkenyl resorcinols) in the methanol, hexane and acetone extracts, respectively with Mangifera indica as major plant source. Three triterpenes, lupeol, lupenone, 27-hydroxymangiferonic acid and an ester of fatty acid heptadecyl butanoate were isolated and characterized. The methanol, acetone and hexane extracts (200-600 mg kg–1) dose-dependently prevented the formation of ethanol-induced gastric lesions (percentage of inhibition, 61, 54 and 55%, respectively, at the dose of 600 mg kg–1). Increasing doses of the extracts inhibited pylorus ligation-induced lesions by 64.5, 73.1 and 16.2%, respectively for the highest dose but none of them showed antisecretory activity compared with controls. The most further significantly (p<0.01) reduced HCl/ethanol-induced ulcer indices from 4.33+0.32 in cytoprotective (acetone) extract (56.6-73.1% inhibition under highly acidic gastric environments), the controls to 1.25+0.53 and 0.6+0.04 at the dose of 400 and 600 mg kg–1, respectively (percentage of inhibition: 71-86%). Furthermore, upon pretreatment of the rats with indomethacin prior to HCl/ethanol, the acetone extract significantly (p<0.001) decreased ulcer index from 5.55+0.73 in the controls to 1.89+0.15 at the dose of 600 mg kg–1. Although pretreatment with indomethacin reduced the protective effect of the acetone extract by 23-27% and cytoprotection remained high (62-66% inhibition). The cytoprotective action of the most active (acetone) extract may involve the mediation of endogenous prostaglandins.
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How to cite this article:
Tamfu Alfred Ngenge, Domgnim Mokam Elisabeth Carol, Talla Emmanuel, Tan Paul Vernyuy, Mbafor Tanyi Joseph, Milena Popova and Vassya Bankova, 2016. Chemical Constituents and Anti-ulcer Activity of Propolis from the North-West Region of Cameroon. Research Journal of Phytochemistry, 10: 45-57.
URL: https://scialert.net/abstract/?doi=rjphyto.2016.45.57
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