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Research Journal of Phytochemistry
  Year: 2014 | Volume: 8 | Issue: 3 | Page No.: 92-101
 
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Evaluation of the Antibacterial Potential, Preliminary Phytochemical Screening of Medicinal Plant against Plant Pathogen

V. Nithya Devi and S. Pradeep Kumar

Abstract:
Vitex negundo L. belongs to family Verbenaceae is a hardy plant, flourishing mainly in the Indian subcontinent. All parts of the plant, from root to fruit, possess a multitude of phytochemical secondary metabolites which impart an unprecedented variety of medicinal uses to the plant. In the present study, the dried leaves of the Vitex negundo were powdered and extracted using cow urine extract, aqueous, hexane, chloroform, ethyl acetate and alcohol. The extracts were screened for antibacterial activity against Xanthomonas campestris pv citri. All the extracts and fractions were effective and showed 8 to 16 mm zone of inhibition. The chloroform extract (14 mm) and cow urine extract (16 mm) showed excellent antibacterial activity. The MIC values were determined by agar dilution method. Results of hexane and chloroform extracts had lowest MIC value 0.4 μg mL-1. Aqueous ethyl acetate and cow urine extract had 1.85 μg mL-1, respectively. Alcohol extracts had highest MIC value 5.55 μg mL-1. Preliminary qualitative phytochemical analysis showed the presence of alkaloids, steroids, flavones, saponins, tannins, sugar, terpinoids, coumarins, phenols, phlobotanins, phytosterol and quantitative estimation evaluated that cow urine extract of V. negundo possess highest flavonoids (1.25 mg kg-1) among which alkaloids, tannins, terpinoids contents were low. Aqueous and chloroform extracts had very low quantity of the phytochemicals. The main aim of this product development is to provide employment to the rural youth and to use safe disinfectant of cleaning floors etc.
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How to cite this article:

V. Nithya Devi and S. Pradeep Kumar, 2014. Evaluation of the Antibacterial Potential, Preliminary Phytochemical Screening of Medicinal Plant against Plant Pathogen. Research Journal of Phytochemistry, 8: 92-101.

URL: https://scialert.net/abstract/?doi=rjphyto.2014.92.101

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