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Research Journal of Phytochemistry
  Year: 2014 | Volume: 8 | Issue: 3 | Page No.: 119-126
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Antioxidant Properties of Methanolic Extracts of Boerhavia diffusa

Pallavi Sharma, Richa Bhardwaj, Ankita Yadav and R.A. Sharma

Natural antioxidants present are responsible for inhibiting or preventing the deleterious consequences of oxidative stress. Free radical scavengers are compounds like polyphenols, flavonoids and phenolic compounds. The present study was carried out to evaluate the antioxidant activity and phytochemical constituents of Boerhaavia diffusa L., a herbaceous member of the family Nyctaginaceae from methanolic extracts of different plant parts. Methanolic extracts of leaves, stems and roots were prepared and screened for in vitro antioxidant activities by using peroxidase assay, Ferric Reducing Ability of Plasma (FRAP), lipid peroxidation assay (LPO), ABTS and the quantitative estimation of the total phenolics as Gallic Acid Equivalent (GAE) per gram dry weight and total flavonoid as Quericitin Equivalent (QE) per gram dry weight. Maximum total phenolic content was recorded in leaves (24.5±1.703 mg GAE g-1 DW) While maximum total flavonoid content was found in leaves (79.86±3.751 mg QE g-1 DW). Leaves methanolic extract showed maximum radical scavenging acitvity for FRAP (96.664 Mm L-1 g-1), while ethyl acetate and hexane extract of stems showed minimum activity for FRAP. Leaves showed maximum radical scavenging activity for LPO (26.66 MDA g-1 DW) and peroxidase (0.383 Mm min-1 g-1 DW), a good correlation with the total phenolic and flavonoidal content of plant is seen. It signifies that the plant-derived phenolics and flavonoids represents good sources of natural antioxidants. It is seen that this plant exhibits significant antioxidant activity.
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Pallavi Sharma, Richa Bhardwaj, Ankita Yadav and R.A. Sharma, 2014. Antioxidant Properties of Methanolic Extracts of Boerhavia diffusa. Research Journal of Phytochemistry, 8: 119-126.




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