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Research Journal of Phytochemistry
  Year: 2014 | Volume: 8 | Issue: 1 | Page No.: 1-8
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Phytochemical Screening and Preliminary TLC Characterization of Alkaloids in Sabicea brevies Root

C.C. Ogbuanu, R.C. Ehiri and S.P.I. Ogah

Sabicea brevipes (susu) plant root in Oghe Community medicine is of value in the management of male erectile dysfunction. The objective of the study was to evaluate the phytochemicals and the number of alkaloids present. Phytochemical screening of n-hexame, ethyl acetate and methanol crude extract of Sabicea brevipes (susu) root were evaluated. The extracts were subjected to qualitative chemical analysis for identification of various secondary metabolites or active principles. The extracts revealed the presence of steroids, alkaloids, gylcosides, saponins, tannins, triterpenoids, antracene, flavonoids and volatile oils. The Result of this study x-rayed valuable evidence in support of Sabicea brevipes plant as an enhancer of sexual function in men. Phyto-chemical investigation resulted in isolation and TLC identification of several alkaloids using five solvent system. Results showed that the yields of the extract were 0.43% and 0.29% for morphine and non-morphine alkaloids, respectively. There were seven spots observed in the chromatogram developed for morphine alkaloids while five spots were seen for the other (non-morphine) alkaloids in the various solvent system used, visualizing with dragendoffs location reagent. The result shows that the solvent systems A chloroform: ethanol (9:1), C methanol Ammonia (99:1) and E chloroform: Methanol: Ammonia (60:60:1) contain one alkaloid each for morphine and non morphine alkaloids with RF values 0.64 and 0.55 (solvent system A); 0.55 and 0.27 (solvent system C) and 0.55 and 05.2 (solvent system E)s, respectively. While solvent systems B chloroform: ethanol (85:15) had two spots for morphine alkaloids with RF value 0.34 and 0.84 and solvent system D, Methanol: ethyl acetate: ammonia (9.5:81:9.5) also had two spots for morphine alkaloids with RF of 0.27 and 0.95 and two spots for non-morphine alkaloid with RF 0.60 and 0.95, respectively. These results support the claim about the use of this herb in folk medicines.
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How to cite this article:

C.C. Ogbuanu, R.C. Ehiri and S.P.I. Ogah, 2014. Phytochemical Screening and Preliminary TLC Characterization of Alkaloids in Sabicea brevies Root. Research Journal of Phytochemistry, 8: 1-8.

DOI: 10.17311/rjphyto.2014.1.8




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