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Research Journal of Phytochemistry
  Year: 2012 | Volume: 6 | Issue: 3 | Page No.: 84-91
DOI: 10.3923/rjphyto.2012.84.91
HPLC/DAD Profile and Determination of Total Phenolics, Flavonoids, Tannins and Alkaloids Contents of Scutia buxifolia Reissek Stem Bark
A.A. Boligon, T.F. De Brum, J.K. Frolhich, A.L.F. Froeder and M.L. Athayde

Abstract:
The species Scutia buxifolia Reissek, belongs to Rhamnaceae family and is popularly known in Brazil as coronilha. In this study, total phenolics, flavonoid, tannin and alkalois contents from the stem bark of the plant were investigated. Additionally, HPLC/DAD analysis of ethyl acetate and butanolic fractions revealed six active phenolic compounds (gallic, chlorogenic and caffeic acids; and quercetin, rutin and kaempferol). Total phenolics were measured using folin ciocalteau and total flavonoid, using aluminium chloride. HPLC/DAD profiles were performed using gradient system. The stem bark of S. buxifolia exhibited as a large content of phenolic compounds and flavonoids. Total phenolic varied from 323.47±2.62 to 141.09±0.71 mg g-1, the contents of flavonoids ranged from 145.72±0.27 to 100.37±0.56 mg g-1, tannin and alkaloids varied from 176.70±0.24 to 66.67±0.17 mg g-1 and 3.07±1.13 to 0.28±0.19 mg g-1, respectively. The species S. buxifolia exhibited high phenolics contents, being ethyl acetate and butanolic the most active fractions. Several bioactive phenolics were identified and quantified for the first time in these fractions.
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How to cite this article:

A.A. Boligon, T.F. De Brum, J.K. Frolhich, A.L.F. Froeder and M.L. Athayde, 2012. HPLC/DAD Profile and Determination of Total Phenolics, Flavonoids, Tannins and Alkaloids Contents of Scutia buxifolia Reissek Stem Bark. Research Journal of Phytochemistry, 6: 84-91.

DOI: 10.3923/rjphyto.2012.84.91

URL: https://scialert.net/abstract/?doi=rjphyto.2012.84.91

 
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