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Research Journal of Phytochemistry
  Year: 2012 | Volume: 6 | Issue: 3 | Page No.: 61-74
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Influence of Different Extraction Parameters on Antioxidant Properties of Carica papaya Peel and Seed

L.Y. Ng, Y.K. Ang, H.E. Khoo and H.S. Yim

This study aimed to determine the effect of extraction media, solvent-to-water ratio and extraction time on Total Phenolics (TP) and antioxidant activities of the peel and seed of Carica papaya variety Sekaki. TP was determined by Folin-Ciocalteu’s method, while antioxidant activities were evaluated using DPPH radical scavenging ability and Ferric Reducing/antioxidant Power (FRAP) assays. The ideal extraction conditions for papaya peel were 90% acetone (v/v) and 60 min extraction, which had the highest TP (15.18±0.07 μg mL-1) expressed as gallic acid equivalents (GAE) in extract. The highest antioxidant activities measured using DPPH and FRAP assays were 37.3±1.2% and 19.70±0.47 μg mL-1 expressed as Trolox Equivalents (TE) in extract, respectively. Extraction with deionised water for 120 min was the best extraction condition for papaya seed, with the highest TP of 6.75±0.08 μg mL-1 and highest DPPH and FRAP of 57.3±0.4% and 16.05±0.14 μg mL-1, respectively. Overall, based on the ideal extraction conditions chosen, optimum level of TP and antioxidant activities were obtained in papaya peel and seed extracts. The selected extraction conditions could be used for further studies and functional food product development.
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How to cite this article:

L.Y. Ng, Y.K. Ang, H.E. Khoo and H.S. Yim, 2012. Influence of Different Extraction Parameters on Antioxidant Properties of Carica papaya Peel and Seed. Research Journal of Phytochemistry, 6: 61-74.




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