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Research Journal of Phytochemistry
  Year: 2010 | Volume: 4 | Issue: 2 | Page No.: 78-86
DOI: 10.3923/rjphyto.2010.78.86
Bioassay-Guided Isolation and Identification of Antibacterial and Antifungal Component from Methanolic Extract of Green Tea Leaves (Camellia sinensis)
Devjani Chakraborty and Sunanda Chakraborti

Abstract:
The antibacterial activity of methanolic extract of green tea leaves (Camellia sinensis; Family: Theaceae) was studied on four different bacteria namely Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus and two fungi of Aspergillus species. Antibacterial activity was assigned by measuring the Zone of Inhibition (ZOI) as well as Minimum Inhibitory Concentration (MIC) at four different concentrations of the methanolic extract (10, 25, 50 and 100 mg mL-1) and erythromycin (10 mg mL-1) which was taken as the antibiotic control. Preliminary screening was done to find out the phytochemical components present in the extract. Thin Layer Chromatography (TLC) was performed to separate and isolate the bioactive components present in the extract. Further bioassay-guided fractionation of the methanolic extract with the help of column chromatography was performed using a stepwise gradient with Chloroform: Methanol against the bacteria. The fraction which gave us the best result was further taken for the HPTLC for the identification of the bioactive compound which was identified as catechin. Zone of inhibition was observed in most of the organism. Alcoholic extract of the leaves of Camellia sinensis was found to be most effective against Bacillus cereus.
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How to cite this article:

Devjani Chakraborty and Sunanda Chakraborti, 2010. Bioassay-Guided Isolation and Identification of Antibacterial and Antifungal Component from Methanolic Extract of Green Tea Leaves (Camellia sinensis). Research Journal of Phytochemistry, 4: 78-86.

DOI: 10.3923/rjphyto.2010.78.86

URL: https://scialert.net/abstract/?doi=rjphyto.2010.78.86

 
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