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Research Journal of Phytochemistry
  Year: 2010 | Volume: 4 | Issue: 4 | Page No.: 242-247
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Phytochemical Screening and High Performance TLC Analysis of Some Cucurbits

M. Irshad, Irfan Ahmad, H.C. Goel and M. Moshahid A. Rizvi

Fruit extracts of Lagenaria siceraria (Molina) Standl., Luffa cylindrica (Linn) Roem. and Cucurbita maxima (Duchesne) Lam. was carried out for the phytochemical screening and High Performance TLC analysis to ascertain relative relationship between active compounds. Present study revealed the presence of alkaloids, flavonoids, saponins and steroids in moderate concentration. High Performance TLC on Silica gel 60 F254 TLC aluminium sheet was used to separate the active compound present in the extracts. TLC sheet was scanned at 200 nm and revealed that the retention factor (Rf), 0.05 and 0.26 of active compounds were common in all three vegetables. Another two active compounds of Rf 0.40 and 0.63 were common in Lagenaria siceraria and Luffa cylindrica. However, Rf 0.20 was common in Lagenaria siceraria and Cucumis maxima. Lagenaria siceraria showed highest peaks, having 44.4% paired affinity with Luffa cylindrica and 28.6% with Cucumis maxima, respectively.
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How to cite this article:

M. Irshad, Irfan Ahmad, H.C. Goel and M. Moshahid A. Rizvi, 2010. Phytochemical Screening and High Performance TLC Analysis of Some Cucurbits. Research Journal of Phytochemistry, 4: 242-247.




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