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Research Journal of Phytochemistry
  Year: 2008 | Volume: 2 | Issue: 1 | Page No.: 1-9
 
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HPTLC Method for Estimation of Ellagic Acid and Gallic Acid in Triphala churanam Formulations

N.S. Jeganathan and K. Kannan

Abstract:
Churanams are important group of formulations used by traditional physicians to treat various types of diseases. Triphala churanam, as per Siddha literature is used for the treatment of wounds and local ulcers. In the present study, an attempt has been made to develop a HPTLC (High Performance Thin Layer Chromatography) method of quantitative estimation of marker compounds, ellagic acid and gallic acid in laboratory prepared authentic formulation and a commercial formulation of Triphala churanam. The two formulations were subjected to methanol and ethyl acetate extractions by using Soxhlet apparatus. Ellagic acid and gallic acid were quantified in the above two extracts by using HPTLC. The detection and quantification were performed at a wavelength of 280 nm. The laboratory formulation was found to contain 0.201% w/w of ellagic acid and 0.656% w/w of gallic acid in methanol extract while it shows 0.573% w/w of ellagic acid and 2.664% w/w of gallic acid in the ethyl acetate extract. The commercial formulation shows 0.058% w/w of ellagic acid and 0.573% w/w of gallic acid in methanol extract and 0.422% w/w of ellagic acid and 1.637% w/w of gallic acid in ethyl acetate extract. Linearity studies indicated that ellagic acid and gallic acid were in the linear range of 125-500 ng and 1.25-5.00 μg, respectively, while the % recovery studies revealed a recovery of 99.2% w/w of ellagic acid and 98.13% w/w of gallic acid, thus proving the accuracy and precision of the analysis. Since this method resolves and quantifies ellagic acid and gallic acid effectively, it can be used to quantify the concentration of both the active principles in the herbal formulations.
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How to cite this article:

N.S. Jeganathan and K. Kannan, 2008. HPTLC Method for Estimation of Ellagic Acid and Gallic Acid in Triphala churanam Formulations. Research Journal of Phytochemistry, 2: 1-9.

URL: https://scialert.net/abstract/?doi=rjphyto.2008.1.9

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