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Research Journal of Phytochemistry
  Year: 2007 | Volume: 1 | Issue: 2 | Page No.: 79-82
DOI: 10.3923/rjphyto.2007.79.82
Spectrophotometric Determination of Total Alkaloids in Peganum harmala L. Using Bromocresol Green
Shamsa Fadhil , Monsef Hamid Reza , Ghamooshi Rouhollah and Verdian Rizi Mohammad Reza

Abstract:
A simple spectrophotometric method is described for determination of total alkaloids based on the reaction with Bromocresol Green (BCG). A yellow complex forms and is easily extractable by chloroform at pH 4.7. The absorbance of the complex obeys Bear’s law over the concentration range of 4-13 μg atropine per mL of chloroform. This procedure can be carried out in the presence of other compounds without interference.
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How to cite this article:

Shamsa Fadhil , Monsef Hamid Reza , Ghamooshi Rouhollah and Verdian Rizi Mohammad Reza , 2007. Spectrophotometric Determination of Total Alkaloids in Peganum harmala L. Using Bromocresol Green. Research Journal of Phytochemistry, 1: 79-82.

DOI: 10.3923/rjphyto.2007.79.82

URL: https://scialert.net/abstract/?doi=rjphyto.2007.79.82

 
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