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Research Journal of Medicinal Plants

Year: 2021 | Volume: 15 | Issue: 1 | Page No.: 7-17
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Authors

Dennis R.A. Mans

Dennis R.A. Mans

Priya Magali

Awinash Sardjoepersad

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Research Article

Angiogenesis-Interfering Potential of Wound Healing Plants in Subintestinal Blood Vessels of Tg(fli1a:EGFP)y1/+Zebrafish Embryos

Dennis R.A. Mans Dennis R.A. Mans's LiveDNA, Priya Magali and Awinash Sardjoepersad
Background and Objective: Plants are often traditionally used for managing wounds. Angiogenesis is an important event in wound healing. In this study, six traditionally used wound-healing plants from Suriname (South America) have been evaluated for their capacity to stimulate Subintestinal Vessel (SIV) formation in Tg(fli1a: EGFP)y1/+zebrafish embryos. Materials and Methods: Extracts were prepared from Carapa guianensis, Copaifera guyanensis (stembark), Punica granatum (fruit) and Piper betle, Stachytarpheta jamaicensis as well as Uncaria guianensis (leaves). Zebrafish embryos were exposed to the plant e. The P. betle and S. jamaicensis preparations at 100.0 μg mL–1 also produced a decrease in SIV lengths of around 50% but killed more than half of the embryos. Conclusion: The traditional xtracts (10–5 - 100 μg mL–1) in Hank’s solution containing dimethyl sulfoxide 0.1% (v/v) from 8 hrs post-fertilization (hpf) in ovo until 96 hpf ex ovo. Total SIV lengths were quantified using the Axiovision 4.8.1 Image Acquisition and Management Software. The numbers of surviving embryos were also recorded. Data were compared to those found with untreated controls (ANOVA, p<0.05). Results: None of the plant extracts produced greater SIV lengths than controls. However, the C. guianensis extract at 0.01 μg mL–1 produced a decrease of about 40% in SIV length and left about 70% of the embryos unharmeduse of the plants for wound healing may not involve proangiogenic events. However, the C. guianensis stem bark extract may possess antiangiogenic properties. This may impede wound healing but may be useful against conditions associated with excessive angiogenesis.
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Dennis R.A. Mans, Priya Magali and Awinash Sardjoepersad, 2021. Angiogenesis-Interfering Potential of Wound Healing Plants in Subintestinal Blood Vessels of Tg(fli1a:EGFP)y1/+Zebrafish Embryos. Research Journal of Medicinal Plants, 15: 7-17.

URL: https://scialert.net/abstract/?doi=rjmp.2021.7.17

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