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Research Journal of Medicinal Plants
  Year: 2020 | Volume: 14 | Issue: 2 | Page No.: 53-63
DOI: 10.3923/rjmp.2020.53.63
 
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Antibacterial and Cytotoxic Activities of Three Medicinal Plants from Cameroon (Alstonia boonei, Cassia alata and Garcinia lucida) Against Diarrhea

Melogmo Dongmo Yanickkevin, Lunga Paul Keilah , Toghueo Kouipou Rufin Marie, Djague Fred, Dize Darline and Fekam Boyom Fabrice

Abstract:
Background and Objective: Diarrhea is a bacterial disease classified as the third leading cause of death for children under 5. Due to the frequent setbacks that surround the use of first line antibacterials, this thematic outline was designed with the aim of finding future alternative means of treatment on the basis of Cassia alata, Garcinia lucida and Alstonia boonei, medicinal plants used traditionally for the treatment of diarrhea. Materials and Methods: The various plant extracts were obtained by methanol and hydroethanol maceration and their inhibitory potential was evaluated on 5 diarrheal-causing enterobacteria by the broth microdilution method. Phytochemical screening was performed by colorimetric methods. Cytotoxicity was assessed on Vero cell line by the spectrophotometric method. The effects of the extracts on bacterial (S. flexneri) membrane destabilization and nucleotide leakage was evaluated spectrophotometrically, while the effect on loss of salt tolerance and time kill kinetics was done by enumerating colonies after treatment. Results: MIC values ranged from 500-1000 μg mL1. Phytochemical screening revealed the presence of flavonoids, phenols, anthocyanins, glycosides, quinones and steroids. The CC50 values ranged from 357.9±10.818 to 161.7±65.195 μg mL1. Methanolic extract of C. alata leaves showed its ability to destabilize the S. flexneri membrane as well as foster loss of salt tolerance. The time kill kinetics of S. flexneri showed a bacteriostatic effect up to 8h with the methanolic extracts of leaves of C. alata. Conclusion: The overall results support the traditional use of these plants and show that they could serve as potent sources of non-cytotoxic antidiarrheal phytomedicines.
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How to cite this article:

Melogmo Dongmo Yanickkevin, Lunga Paul Keilah, Toghueo Kouipou Rufin Marie, Djague Fred, Dize Darline and Fekam Boyom Fabrice, 2020. Antibacterial and Cytotoxic Activities of Three Medicinal Plants from Cameroon (Alstonia boonei, Cassia alata and Garcinia lucida) Against Diarrhea. Research Journal of Medicinal Plants, 14: 53-63.

DOI: 10.3923/rjmp.2020.53.63

URL: https://scialert.net/abstract/?doi=rjmp.2020.53.63

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