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Research Journal of Medicinal Plants
  Year: 2019 | Volume: 13 | Issue: 3 | Page No.: 81-88
DOI: 10.3923/rjmp.2019.81.88
 
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Membrane Stabilization Activity of Amino Acids Rich Chromatography Fractions from Pleurotus pulmonarius (Fr.) Quel. (Pleurotaceae)

E.D. Ahanonu, O.E. Afieroho , B.O. Okonkwo and K.A. Abo

Abstract:
Background and Objective: Amino acids are not just important for protein synthesis but are important for whole body homeostasis. Mushrooms aside being used for food and for medicine are also good sources of amino acids that can be used as a nutraceuticals. This study evaluated the Aqueous Ethanol Extract (AEE) of the defatted Pleurotus pulmonarius and its amino acids containing fractions for their Membrane Stabilization (MS) properties. Materials and Methods: Air dried fruiting bodies (150 g) of P. pulmonarius were defatted with n-hexane and dichloromethane successively prior to extraction with 80% aqueous ethanol. Fractionation of AEE was done by using column chromatography and the amino acids derivative rich fractions identified using TLC with ninhydrin as spray reagent. In vitro MS assay was done using the heat induced human erythrocytes haemolysis model. Results: The AEE afforded 2 amino acids derivative containing fractions T2 and T3 in addition to T1 devoid of amino acids constituents. Trend in MS activity (IC50 μg mL1 ), AEE 524.8)>T2 (403.4 )>T3 (40.5)>Aspirin (6.8)>Indomethacin (0.37) was obtained. The TLC analysis for T2 has amino acids at Rf 0.23 similar to that obtained for glycine in addition to two other amino acids at Rf 0.28 and 0.38. T3 have two amino acids spot at Rf 0.08 and 0.13. Conclusion: The MS activity justified Pleurotus pulmonarius as a potential source of agents for the development of nutraceutical and drug lead compounds for the management of diseases associated with inflammation and oxidative stress.
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How to cite this article:

E.D. Ahanonu, O.E. Afieroho, B.O. Okonkwo and K.A. Abo, 2019. Membrane Stabilization Activity of Amino Acids Rich Chromatography Fractions from Pleurotus pulmonarius (Fr.) Quel. (Pleurotaceae). Research Journal of Medicinal Plants, 13: 81-88.

DOI: 10.3923/rjmp.2019.81.88

URL: https://scialert.net/abstract/?doi=rjmp.2019.81.88

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