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Research Journal of Medicinal Plants
  Year: 2018 | Volume: 12 | Issue: 1 | Page No.: 33-40
DOI: 10.3923/rjmp.2018.33.40
 
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Effect of Desmodium velutinum Stem Bark Methanolic Extract on Some Antioxidant Enzymes and Vitamins in Acetaminophen-intoxicated Rats

Nkwocha Chinelo Chinenye , Nworah Florence Nkechi , Okagu Innocent, Obiesie Adaoma, Eze Nnenna, Eze Precious and Nwagwe Ruth Onyinyechi

Abstract:
Background and Objective: This study evaluated phytoconstituents of the methanol extract of Desmodium velutinum (D. velutinum) stem bark and the effect of the extract on the antioxidant status and malondialdehyde (MDA) of acetaminophen-intoxicated rats. Materials and Methods: Twenty four male Wistar albino rats were used for this study and were divided into six groups of four rats each: Group 1 rats were normal control, group 2 rats were intoxicated with acetaminophen (APAP), group 3 rats were acetaminophen-intoxicated and administered with standard drug silymarin (100 mg kg–1 b.wt.), rats in groups 4, 5 and 6 were acetaminophen-intoxicated and pre-treated with methanol extract of Desmodium velutinum (MEDV) (100, 200 and 500 mg kg–1 b.wt., respectively). Phytochemical analyses, activities of glutathione-S-transferase, catalase (CAT), glutathione peroxidase, glutathione reductase (G-Red), reduced glutathione (GSH) and antioxidant vitamins (vitamins A, C and E) were determined using standard methods. Results: Phytochemical analysis revealed the presence of alkaloids, saponins, flavonoids, tannins, glycosides, steroids, carotenoids, anthocyanins, terpenoids and phenols. Rats in group 2 showed a significant (p<0.05) decrease in levels of superoxide dismutase (SOD), CAT, G-Red and GST, GSH and vitamins A and C compared to those in group 1 (normal control). Groups 4, 5 and 6 rats which were acetaminophen-induced and treated with different doses of MEDV, showed a significant (p<0.05) increase in the levels of SOD, catalase, glutathione transferase and glutathione reductase and levels of reduced glutathione when compared to rats in group 2. There was also a significant increase in the vitamins A and C levels of rats in groups 4, 5 and 6 compared to those in group 2. A significantly (p<0.05) higher MDA level was observed in group 2 rats compared to those in the treated and normal control groups. Conclusion: The result of this study suggests that the methanol extract of Desmodium velutinum stem bark contains some bioactive compounds that possess antioxidant properties and reduces the concentration of lipid peroxidation product (MDA) in acetaminophen-intoxicated rats.
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How to cite this article:

Nkwocha Chinelo Chinenye, Nworah Florence Nkechi, Okagu Innocent, Obiesie Adaoma, Eze Nnenna, Eze Precious and Nwagwe Ruth Onyinyechi, 2018. Effect of Desmodium velutinum Stem Bark Methanolic Extract on Some Antioxidant Enzymes and Vitamins in Acetaminophen-intoxicated Rats. Research Journal of Medicinal Plants, 12: 33-40.

DOI: 10.3923/rjmp.2018.33.40

URL: https://scialert.net/abstract/?doi=rjmp.2018.33.40

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