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Research Journal of Medicinal Plants
  Year: 2017 | Volume: 11 | Issue: 1 | Page No.: 25-31
DOI: 10.3923/rjmp.2017.25.31
 
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Combretum farinosum Extract Toxicity to Skin and Lung Cell Lines as Measured by the Methylthiazoletetrazolium Assay

Elester Williams, Francisco Javier Rendon-Sandoval and Alfred Addo-Mensah

Abstract:
Background: The use of plants from the Combretaceae family in medicine has been of much interest to scientists. However, Combretum farinosum has little published research on its pharmacological properties, unlike many of its extensively studied congenerics. Materials and Methods: Combretum farinosum roots, fruits, leaves and stems were sequentially extracted via Soxhlet extraction using petroleum ether, acetone and ethanol solvents. A 3 day Wallert and Provost Lab™ 96-well plate MTT cell proliferation assay was performed on LL47 lung fibroblasts, H69 small lung cell carcinoma and BJ fetal foreskin cell lines. Results: Combretum farinosum roots petroleum ether extract showed the strongest anti-proliferation activity of all the extracts against the normal cell lines (IC50 of 0.504 mg mL–1 for the BJ cells and 0.608 mg mL–1 for the LL47 cells). Combretum farinosum roots petroleum ether extract also showed the strongest anti-proliferation activity of all the extracts against the only cancer cell line tested (IC50 = 0.642 mg mL–1). Conclusion: Both the roots petroleum ether and roots acetone extracts may have potential use in targeting diseased non-cancerous tissue (e.g., benign tumors) due to their cytotoxicity to normal cell lines. Finally, the roots petroleum ether extract may be the most promising extract for potential use as an anticancer drug if active compounds can be more thoroughly isolated.
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How to cite this article:

Elester Williams, Francisco Javier Rendon-Sandoval and Alfred Addo-Mensah, 2017. Combretum farinosum Extract Toxicity to Skin and Lung Cell Lines as Measured by the Methylthiazoletetrazolium Assay. Research Journal of Medicinal Plants, 11: 25-31.

DOI: 10.3923/rjmp.2017.25.31

URL: https://scialert.net/abstract/?doi=rjmp.2017.25.31

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