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Research Journal of Medicinal Plants
  Year: 2016 | Volume: 10 | Issue: 4 | Page No.: 314-319
DOI: 10.3923/rjmp.2016.314.319
 
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Antioxidant Properties of Leaf, Twig and Calli Extracts of Neolamarckia cadamba (Roxb.) Bosser in Sri Lanka

A.M. DonPaul, S.R. Weerakoon and S. Somaratne

Abstract:
Neolamarckia cadamba (Roxb.) Bosser poses a threat of extinction due to felling for timber, large-scale sample collections for herbal formulations in traditional medicine and research purposes. Use of calli as the source of secondary metabolites instead of destructive sampling conserves N. cadamba for future generations. Objectives of the present study were to evaluate antioxidant activity of natural extracts of leaf and twigs of N. cadamba, through optimization of calli induction of leaf and internode explants and a comparison of calli and plant extracts for their antioxidant activity. Calli were induced from tender leaves and internodes using two out of ten NAA and BAP hormone combinations; NAA 5.0 mg L–1: BAP 0.5 mg L–1 and NAA 2.5 mg L–1: BAP 3.0 mg L–1. Methanolic crude extracts of mature leaves, twigs and calli were obtained and the extracts were screened for antioxidant activity using in vitro assays; Total Phenol Content (TPC) assay, DPPH assays and plasmid DNA nicking assay. The TPC was highest in twigs than leaves of natural plant and call extracts which in turn indicated increase antioxidant activity. Calli obtained from leaves and internodes are of important as a potential source of bioactive compounds showing the capability of scavenge the diverse free radicals in different systems and as potential therapeutic agents for treating radical-related pathogenic cell damages. Further studies focusing the enhancement of production of secondary metabolites in calli of N. cadamba exposing to different stress conditions is a prerequisite to produce important bioactive compounds in higher concentrations.
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How to cite this article:

A.M. DonPaul, S.R. Weerakoon and S. Somaratne, 2016. Antioxidant Properties of Leaf, Twig and Calli Extracts of Neolamarckia cadamba (Roxb.) Bosser in Sri Lanka. Research Journal of Medicinal Plants, 10: 314-319.

DOI: 10.3923/rjmp.2016.314.319

URL: https://scialert.net/abstract/?doi=rjmp.2016.314.319

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