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Research Journal of Medicinal Plants
  Year: 2014 | Volume: 8 | Issue: 1 | Page No.: 32-40
DOI: 10.3923/rjmp.2014.32.40
 
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Potentiation of Isoniazid Efficacy against Isoniazid-resistant Mycobacteria Strains

P. Erasto, C. Marciale, J.J. Omolo and C.J. Hamilton

Abstract:
This study aimed to isolate antimycobacterial compounds from the leaves of Hallea rubrostipulata, a medicinal plant used in the treatment of respiratory tract infection in Karagwe district, Tanzania. It further aimed to investigate the ability of compounds to break and/or circumvent the resistance of Mycobacterium madagascariense (MM) and Mycobacterium inducus pranii (MIP) against isoniazid. The isolation of compounds from the leaves extracts of H. rubrostipulata was achieved using various chromatographic techniques. Two indole alkaloids namely; Mitraphylline and isomitraphylline were isolated and their structures were deduced using Nuclear Magnetic Resonance (NMR) and Mass Spectrophotometry (MS) analyses. The antimycobacterial activity of indole alkaloids against isoniazid resistant strains namely; Mycobacterium madascariense (MM) and Mycobacterium indicus pranii (MIP) was evaluated using two folds broth microdilution method. The drug combination assay was done by blending ½ to 1/16 of alkaloid’s MIC values with those of isoniazid (recorded previously against M. tuberculosis, strain Mtb H37Rv). The potential cytotoxicity activity of alkaloids was evaluated using Brine Shrimp Toxicity assay (BST). The indole alkaloids exhibited moderate antimycobacterial activity against test organisms. Mitraphylline had MIC values of 0.8 and 0.4 mg mL-1 against MM and MIP, respectively, while isomitraphylline had MIC values of 0.8 mg mL-1 against all organisms. In the drug combination assay, all compounds potentiated the activity of isoniazid against the two mycobacteria strains. The cytotoxicity assay revealed that, the two indole alkaloids are not toxic to shrimps. These results confirmed why H. rubrostipulata has for many years been used in the treatment of respiratory tract infections.
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How to cite this article:

P. Erasto, C. Marciale, J.J. Omolo and C.J. Hamilton, 2014. Potentiation of Isoniazid Efficacy against Isoniazid-resistant Mycobacteria Strains. Research Journal of Medicinal Plants, 8: 32-40.

DOI: 10.3923/rjmp.2014.32.40

URL: https://scialert.net/abstract/?doi=rjmp.2014.32.40

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