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Research Journal of Medicinal Plants
  Year: 2013 | Volume: 7 | Issue: 1 | Page No.: 68-76
DOI: 10.3923/rjmp.2013.68.76
 
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Facile Isolation and Purification of Thailandine, a Biologically Active Oxoaporphine Alkaloid, from Stephania venosa Leaves using Ion-pair Liquid-liquid Extraction
Nitirat Chimnoi, Nisachon Khunnawutmanotham, Pitchayada Feungfuloy, Kittima Chatrewongwan and Supanna Techasakul

Abstract:
Thailandine is a quaternary oxoaporphine alkaloid isolated from Stephania venosa. This compound has shown highly potent and broad range of biological effects, including cytotoxic, antimicrobial, antimalarial (Plasmodium falciparum, K1 strain) and antitubercular (Mycobacterium tuberculosis H37Ra) activities. In this study, ion-pair extraction was applied to isolate and purify thailandine. The proposed method involved the extraction of dried S. venosa leaves with methanol. After solvent removal, the crude extract was dissolved in water and partitioned thrice with dichloromethane (1:1 v/v) to remove non and semi-polar substances. Dichloromethane was discarded and a volatile ion-pair reagent, trifluoroacetic acid, was added to the aqueous layer to form an ion-pair substance with thailandine. The ion-pair product was then transferred to the dichloromethane layer. After dichloromethane removal, thailandine was obtained as a red precipitate. Purity and identification were determined using NMR and ESI-HRMS. The results indicated that the proposed method could provide thailandine with high purity. The conditional optimizations showed that using 24 h maceration with methanol at room temperature for extraction and 1% v/v trifluoroacetic acid for ion-pair forming provided thailandine with the optimum yield of 0.18% based on dried weight. This study demonstrated the successful utilization of the ion-pair liquid-liquid extraction for thailandine isolation and purification.
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How to cite this article:

Nitirat Chimnoi, Nisachon Khunnawutmanotham, Pitchayada Feungfuloy, Kittima Chatrewongwan and Supanna Techasakul, 2013. Facile Isolation and Purification of Thailandine, a Biologically Active Oxoaporphine Alkaloid, from Stephania venosa Leaves using Ion-pair Liquid-liquid Extraction. Research Journal of Medicinal Plants, 7: 68-76.

DOI: 10.3923/rjmp.2013.68.76

URL: https://scialert.net/abstract/?doi=rjmp.2013.68.76

 
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