Several literatures exist on the efficacy of Cyphostemma glaucophilla in the treatment of diverse ailment ranging from malnutrition disorders to systemic diseases such as hypertension and kidney problems. Effect of aqueous leave extract of Cyphostemma glaucophilla was investigated on Carbon tetrachloride induced hepatotoxicity by evaluating its biochemical activities on Aspartate Amino Transferase (AST), Alanine Amino Transferase (ALT), Alkaline Phosphatase (ALP), Bilirubin and creatinine. The negative control group A were served daily doses of saline (0.85% NaCl; 5 mL kg-1) positive control group B received (0.85% NaCl; 5 mL kg-1 and a single concentration 5 mL kg-1 of CCl4) while groups C, D, E and F were given 5.0, 10.0, 15.0, 20.0 mg kg-1 body weight of extract using stomach tubes for 7 days, respectively. CCl4 (5 mL kg-1) single concentration was administered to groups B, C, D, E and F on the 8th day however, extract administration continued for groups C, D, E and F on day 9 and 10. The activities of the enzyme markers in the positive control group were observed to increase significantly (p<0.05) when compared with the negative control and the test groups C, D, E and F. The test groups administered varying doses of extract after exposure to CCl4 decreased significantly (p<0.05) and in a dose dependent manner the activities of ALT, AST and ALP. There was also a significant (p<0.05) dose dependent decrease in the level of unconjugated, conjugated bilirubin and creatinine. It is observed, that Cyphostemma glaucophilla among its numerous pharmacological properties may serve as a cheap alternative in maintaining liver and kidney disorders.