Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
Research Journal of Medicinal Plants
  Year: 2011 | Volume: 5 | Issue: 6 | Page No.: 728-737
DOI: 10.3923/rjmp.2011.728.737
Preliminary Anticancer Screening and Standardization of some Indigenous Medicinal Plants using Cell-biology and Molecular Biotechnology Based Models
S. Kavitha Bagya, P.V. Rajashree and Kishore Gnana Sam

Abstract:
Alcoholic extracts of four Indian medicinal plants Kaempferia galanga (Zingiberacae) Linn. Clerodendrum viscosum (Verbenaceae) Linn., Jatropha curcus (Euphorbiaceae) Linn. and Lens culinaris (Fabaceae) Linn. were subjected to preliminary screening for their antitumor activity. Acute toxicity studies in mice revealed that all the ethanolic extracts were safe up to a dose level of 500, 1000, 2000 mg kg-1 body weight. Preliminary short term anticancer screening, by brine shrimp lethality test, potato disc inhibition and DLA cell line assay, proved that K. galanga, exhibited significant antitumor activity and it was therefore, selected as a candidate plant for more detailed phytochemical and mechanistic studies. Brine shrimp lethality assay revealed that K. galanga extract inhibited tumor development at a lower concentration LC50 = 684.2 μg mL-1 as compared to 901, 866 and 5436 μg mL-1 for the extracts of C. viscosum, J. curcus and L. culnaris, respectively. Alcoholic extract of K. galanga significantly (p<0.001) inhibited Agrobacterium induced tumors in potato discs with average tumor count of 15, 11, 8.0, 6.0 and 4.8 at concentrations of 10, 20, 30, 40 and 50 μg mL-1, respectively. K. galanga extract regress tumors equipotently to vincristine in Dalton Lymphoma Ascitic (DLA) cell tumor bearing mice. There was a statistically significant (p<0.001) higher mean increase in Percentage Life Span (ILS) in rats treated with K. galanga extract 73.27±10.51 with median value of 69.85% as compared to Vincristine group 53.84±11.94 with median 54.25%. Preliminary phytochemical tests of the candidate plant K. galanga indicated the presence of flavonoids, suggesting a prominent role for them in anticancer activity.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Antitumor Activity of Hexane and Ethyl Acetate Extracts of Tragia involucrata
  •    Hepatoprotective Effect of Cassia auriculata L. Leaf Extract on Carbon Tetrachloride Intoxicated Liver Damage in Wister Albino Rats
  •    Cytotoxicity of the Fungicides Azoxystrobin and Difenoconazole in Root Tips of Allium cepa L.
  •    Comparative Cytotoxicity Study of Six Bioactive Lectins Purified from Pondweed (Potamogeton nodosus Poir) Rootstock on Brine Shrimp
  •    Radical Scavenging Activity, Phenolic Content and Cytotoxicity of Bark and Leaves Extracts of Entada africana Guill. and Perr. (Mimosaceae)
How to cite this article:

S. Kavitha Bagya, P.V. Rajashree and Kishore Gnana Sam, 2011. Preliminary Anticancer Screening and Standardization of some Indigenous Medicinal Plants using Cell-biology and Molecular Biotechnology Based Models. Research Journal of Medicinal Plants, 5: 728-737.

DOI: 10.3923/rjmp.2011.728.737

URL: https://scialert.net/abstract/?doi=rjmp.2011.728.737

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 

       

       

Curve Bottom