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Research Journal of Medicinal Plants
  Year: 2007 | Volume: 1 | Issue: 2 | Page No.: 48-53
DOI: 10.3923/rjmp.2007.48.53
 
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Effect of Plant Growth Regulators on Callus Induction and Regeneration of Bunium persicum (Boiss.) B. Fedtsch

M. Valizadeh , S.K. Kazemi Tabar and G.A. Nematzadeh

Abstract:
The effect of various media and combinations of Plant Growth Regulators (PGRs) on callus induction and shoot regeneration from hypocotyl explant were investigated. Simultaneous callus and shoot regeneration were obtained. The experiment was conducted as a completely randomized design. The highest callus frequency was observed on MS medium containing 0.1 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L-1 2,4-D as well as 2 mg L-1 2,4-D and 0.5 mg L-1 Kinetin (Kin). The best response for shoot regeneration was observed on MS medium containing 1 mg L-1 2,4-D. MS medium supplemented with 1 mg L-1 2,4-D was the best for callus induction and shoot regeneration simultaneously. The regenerated plantlets were transferred to basal medium to be rooted. However suitable combination of auxins and cytokinins are important for embryogenesis and organogenesis. For the exploitation of in vitro techniques it is essential to optimize the conditions for whole plant regeneration.
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How to cite this article:

M. Valizadeh , S.K. Kazemi Tabar and G.A. Nematzadeh , 2007. Effect of Plant Growth Regulators on Callus Induction and Regeneration of Bunium persicum (Boiss.) B. Fedtsch. Research Journal of Medicinal Plants, 1: 48-53.

DOI: 10.3923/rjmp.2007.48.53

URL: https://scialert.net/abstract/?doi=rjmp.2007.48.53

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