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Research Journal of Medicinal Plants
  Year: 2007 | Volume: 1 | Issue: 2 | Page No.: 42-47
DOI: 10.3923/rjmp.2007.42.47
 
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Regeneration of Plantlets from Embryo Explants of Bunium Persicum (Boiss.) B. Fedtsch

M. Valizadeh , S.K. Kazemi Tabar and G.A. Nematzadeh

Abstract:
A new simple method was developed for regeneration of Parsi Zira. This method yielded a large number of shoots within short period of time (30-40 days) without any sub culturing. The effect of various combinations of Plant Growth Regulators (PGRs) on callus formation and shoot regeneration were investigated on MS medium. Simultaneous callus and root formation and shoot regeneration were obtained. The experiment was conducted in a completely randomized design with 30 treatments and 10 replications per treatment. The best treatment for regeneration was the medium supplemented with 0.1 mg L-1 α-Naphthalene Acetic Acid (NAA) and 2 mg L-1 kinetin (Kin). The highest somatic embryogenesis was obtained in the treatment containing 0.1 mg L-1 NAA and 0.5 mg L-1 Kin. The medium containing 2 mg L-1 NAA and 2 mg L-1 Kin was the best treatment for callus and root induction and regeneration simultaneously.
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How to cite this article:

M. Valizadeh , S.K. Kazemi Tabar and G.A. Nematzadeh , 2007. Regeneration of Plantlets from Embryo Explants of Bunium Persicum (Boiss.) B. Fedtsch. Research Journal of Medicinal Plants, 1: 42-47.

DOI: 10.3923/rjmp.2007.42.47

URL: https://scialert.net/abstract/?doi=rjmp.2007.42.47

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